gentatest

modulates the gentamicin activity against multi-resistant strains and induces nefroprotection

Dioclea violacea lectin modulates the gentamicin activity against multi-resistant strains and induces nefroprotection during antibiotic exposure.

Gentamicin is an aminoglycoside antibiotic used to treat infections of various origins. In the last few decades, the constant use of gentamicin has resulted in increased bacterial resistance and nephrotoxicity in some cases. In this study, we examined the ability of Dioclea violacea lectin (DVL) in modulate the antimicrobial activity of gentamicin and reduce the nephrotoxicity induced by this drug.

 

The minimum inhibitory concentration (MIC) obtained for DVL against all strains studied was not clinically relevant (MIC ≥ 1,024 μg/mL). However, when DVL was combined with gentamicin, a significant increase in antibiotic action was observed against Staphylococcus aureus and Escherichia coli. DVL also reduced antibiotic tolerance in S. aureus during 10 days of continuous treatment.

 

In addition, DVL presented a nephroprotective effect, reducing sodium excretion, N-Gal expression and urinary protein, that are important markers of glomerular and tubular injuries. Taken together, studies of inhibition of hemagglutinating activity, fluorescence spectroscopy and molecular docking revealed that gentamicin can interact with DVL via the carbohydrate recognition domain (CRD), suggesting that the results obtained in this study may be directly related to the interaction of DVL-gentamicin and with the ability of the lectin to interact with glycans present in the cells of the peritoneum.

Antioxidant, anti-inflammatory, and antiapoptotic effects of virgin coconut oil against antibiotic drug gentamicin-induced nephrotoxicity via the suppression of oxidative stress and modulation of iNOS/NF-ĸB/caspase-3 signaling pathway in Wistar rats.

Gentamicin is an effective antibiotic against severe infections; however, its major side effect is oxidative nephrotoxicity. We explored whether virgin coconut oil (VCO) could mitigate gentamicin-induced nephrotoxicity. Rats were fed with VCO-supplemented diet for 16 days against renal toxicity induced by gentamicin (100 mg/kg bw, ip) from Day 11 to 16. Gentamicin caused marked elevated serum urea, uric acid, and creatinine levels, followed by considerable depletion in renal antioxidant enzymes, glutathione (GSH), while the malondialdehyde (MDA) level increased significantly.

 

  • It significantly increased renal cytokines and nitric oxide (NO) levels, confirmed by renal histopathology. The expression of inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-ĸB), and caspase-3 was prominently increased.
  • VCO-supplemented diet significantly modulated the levels of biochemical indices, downregulated the expression of NO, iNOS, NF-ĸB, caspase-3, cytokines, and alleviated histopathological lesions. VCO protects against gentamicin-induced nephrotoxicity; thus, it could be a promising dietary supplement for patients undergoing gentamicin treatment.
  • PRACTICAL APPLICATIONS: Gentamicin is an efficacious clinical antibiotic used against severe infections; however, the robust body of evidence indicates that the nephrotoxic side effect constrained its use. Virgin coconut oil (VCO) is an edible oil with growing human consumption and pharmacological effects.
  • Our study has reported herein, for the first time, that VCO diet prevented the nephrotoxicity of gentamicin. Dietary supplementation of this oil could be beneficial in alleviating the nephrotoxic side effect of gentamicin in patients.
gentatest
gentatest

 

Gentamicin-BSA

80-1453 1 mg
EUR 592
Description: BSA conjugated Gentamicin Hapten

Gentamicin-BSA

80-1454 1 mg
EUR 592
Description: BSA conjugated Gentamicin Hapten

Gentamicin-BSA

80-IG10 25 mg
EUR 325
Description: Conjugated Gentamicin-BSA hapten

Gentamicin-BSA

MBS537343-25mg 25mg
EUR 505

Gentamicin-BSA

MBS537343-5x25mg 5x25mg
EUR 2115

Gentamicin-BSA

MBS7041680-01mg 0.1mg
EUR 135

Gentamicin-BSA

MBS7041680-1mg 1mg
EUR 435

Gentamicin-BSA

MBS7041680-5x1mg 5x1mg
EUR 1915

BSA conjugated Gentamicin Hapten

MBS5308011-1mg 1mg
EUR 930

BSA conjugated Gentamicin Hapten

MBS5308011-5x1mg 5x1mg
EUR 4035

BSA conjugated Gentamicin Hapten

MBS5308012-1mg 1mg
EUR 930

BSA conjugated Gentamicin Hapten

MBS5308012-5x1mg 5x1mg
EUR 4035

Gentamicin-BSA Conjugate

MBS7115390-1mg 1mg
EUR 490

Gentamicin-BSA Conjugate

MBS7115390-5x1mg 5x1mg
EUR 2150

Gentamicin-BSA Conjugate

MBS7115391-1mg 1mg
EUR 490

Gentamicin-BSA Conjugate

MBS7115391-5x1mg 5x1mg
EUR 2150

Gentamicin

EM061-150ST 1 unit
EUR 169.41
Description: Gentamicin

Gentamicin

EM025-150ST 1 unit
EUR 164.38
Description: Gentamicin

Gentamicin

G272 10ml
EUR 70

Gentamicin

HY-A0276A Get quote Ask for price
Description: Gentamicin, an orally active aminoglycoside antibiotic, inhibits the growth of both gram-positive and gram-negative bacteria and to inhibit several strains of mycoplasma in tissue culture. Gentamicin inhibits DNase I with an IC50 of 0.57 mM[1][2][3][4].

Gentamicin

MBS5775176-5mg 5(mg
EUR 915

Gentamicin

MBS5775176-5x5mg 5x5(mg
EUR 3970

Gentamicin

MD025-1PK 1 unit Ask for price
Description: Gentamicin

Gentamicin

MD061-1PK 1 unit Ask for price
Description: Gentamicin

Gentamicin

T25447-10mg 10mg Ask for price
Description: Gentamicin

Gentamicin

T25447-1g 1g Ask for price
Description: Gentamicin

Gentamicin

T25447-1mg 1mg Ask for price
Description: Gentamicin

Gentamicin

T25447-50mg 50mg Ask for price
Description: Gentamicin

Gentamicin

T25447-5mg 5mg Ask for price
Description: Gentamicin

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 76.86

Gentamicin(GM)

AT022 1mg
EUR 1134

Gentamicin(GM)

AG022 1 mg
EUR 627.6

Gentamicin (PE)

MBS6251839-01mL 0.1(mL
EUR 880

Gentamicin (PE)

MBS6251839-5x01mL 5x0.1mL
EUR 3805

Gentamicin (AP)

MBS6247946-01mL 0.1(mL
EUR 880

Gentamicin (AP)

MBS6247946-5x01mL 5x0.1mL
EUR 3805

Gentamycin (Gentamicin)

MBS614836-025mL 0.25mL
EUR 490

Gentamycin (Gentamicin)

MBS614836-5x025mL 5x0.25mL
EUR 2055

Gentamycin (Gentamicin)

MBS615263-025mL 0.25mL
EUR 515

Gentamycin (Gentamicin)

MBS615263-5x025mL 5x0.25mL
EUR 2155

Gentamycin (Gentamicin)

MBS605789-1mg 1mg
EUR 530

Gentamycin (Gentamicin)

MBS605789-5x1mg 5x1mg
EUR 2230

Gentamycin (Gentamicin)

MBS614871-1mg 1mg
EUR 450

Gentamycin (Gentamicin)

MBS614871-5x1mg 5x1mg
EUR 1865

Gentamycin (Gentamicin)

MBS600426-025mg 0.25mg
EUR 580

Gentamycin (Gentamicin)

MBS600426-05mg 0.5mg
EUR 965

Gentamycin (Gentamicin)

MBS600426-5x05mg 5x0.5mg
EUR 4185

Gentamycin (Gentamicin)

MBS603541-05mg 0.5mg
EUR 1285

Gentamycin (Gentamicin)

MBS603541-5x05mg 5x0.5mg
EUR 5640

Gentamicin [OVA]

DAG4468O 1 mg
EUR 520
Description: Synthetic

Gentamicin [KLH]

DAG4484 1mg
EUR 834.75
Description: Synthetic

Gentamicin [HRP]

DAGA-032H 1mg
EUR 834.75
Description: Synthetic

Gentamicin C1a

HY-148286 10 mg
EUR 77.92
Description: Gentamicin C1a is the precursor of the semi-synthetic antibiotic Etimicin, and has antibacterial activity. Gentamicin C1a is the major component of the Gentamicin complex[1][2].

Gentamicin (HRP)

MBS6250887-01mL 0.1(mL
EUR 880

Gentamicin (HRP)

MBS6250887-5x01mL 5x0.1mL
EUR 3805

Gentamicin (APC)

MBS6268799-01mL 0.1mL
EUR 880

Gentamicin (APC)

MBS6268799-5x01mL 5x0.1mL
EUR 3805

Gentamicin (FITC)

MBS6249892-01mL 0.1(mL
EUR 880

Gentamicin (FITC)

MBS6249892-5x01mL 5x0.1mL
EUR 3805

Gentamicin-HRP

65-IG10 1 mg
EUR 525
Description: Conjugated Gentamicin-HRP hapten

Gentamicin-OVA

80-1455 1 mg
EUR 592
Description: OVA conjugated Gentamicin Hapten

Gentamicin-OVA

80-1456 1 mg
EUR 592
Description: OVA conjugated Gentamicin Hapten

Gentamicin-HRP

MBS537743-1mg 1mg
EUR 680

Gentamicin-HRP

MBS537743-5x1mg 5x1mg
EUR 2915

Gentamicin-OVA

MBS7041681-01mg 0.1mg
EUR 135

Gentamicin-OVA

MBS7041681-1mg 1mg
EUR 435

Gentamicin-OVA

MBS7041681-5x1mg 5x1mg
EUR 1915

Gentamicin (Biotin)

MBS6248965-01mL 0.1(mL
EUR 880

Gentamicin (Biotin)

MBS6248965-5x01mL 5x0.1mL
EUR 3805

Gentamicin Sulfate

40710020-1 5 g
EUR 36.51

Gentamicin Sulfate

40710020-2 10 g
EUR 61.99

Gentamicin Sulfate

40710020-3 25 g
EUR 117.28

Gentamicin Sulfate

16637-32 25G
EUR 630

Gentamicin Sulfate

08975-81 1G
EUR 77

Gentamicin Sulfate

08975-94 5G
EUR 266

Gentamicin sulfate

329895 1.0g
EUR 250

Gentamicin sulfate

B1270-1G each
EUR 170.4

Gentamicin sulfate

B1270-25G each
EUR 1136.4

 

Gentamicin, azithromycin and ceftriaxone in the treatment of gonorrhoea: the relationship between antibiotic MIC and clinical outcome.

To investigate the relationship between MIC and clinical outcome in a randomized controlled trial that compared gentamicin 240 mg plus azithromycin 1 g with ceftriaxone 500 mg plus azithromycin 1 g. MIC analysis was performed on Neisseria gonorrhoeae isolates from all participants who were culture positive before they received treatment.

Viable gonococcal cultures were available from 279 participants, of whom 145 received ceftriaxone/azithromycin and 134 received gentamicin/azithromycin. Four participants (6 isolates) and 14 participants (17 isolates) did not clear infection in the ceftriaxone/azithromycin and gentamicin/azithromycin arms, respectively. MICs were determined by Etest on GC agar base with 1% Vitox.

 

The geometric mean MICs of azithromycin, ceftriaxone and gentamicin were compared using logistic and linear regression according to treatment received and N. gonorrhoeae clearance.As the azithromycin MIC increased, gentamicin/azithromycin treatment was less effective than ceftriaxone/azithromycin at clearing N. gonorrhoeae.

 

There was a higher geometric mean MIC of azithromycin for isolates from participants who had received gentamicin/azithromycin and did not clear infection compared with those who did clear infection [ratio 1.95 (95% CI 1.28-2.97)], but the use of categorical MIC breakpoints did not accurately predict the treatment response.

 

The geometric mean MIC of azithromycin was higher in isolates from the pharynx compared with genital isolates.We found that categorical resistance to azithromycin or ceftriaxone in vitro, and higher gentamicin MICs in the absence of breakpoints, were poorly predictive of treatment failure.

 

 

Gentamicin Sulfate

08975-94 5G
EUR 266

Gentamicin sulfate

329895 1.0g
EUR 250

Gentamicin Sulfate

40710020-1 5 g
EUR 36.51

Gentamicin Sulfate

40710020-2 10 g
EUR 61.99

Gentamicin Sulfate

40710020-3 25 g
EUR 117.28

Gentamicin Sulfate

16637-32 25G
EUR 630

Gentamicin sulfate

B1270-1G each
EUR 170.4

Gentamicin sulfate

B1270-25G each
EUR 1136.4

Gentamicin sulfate

B1270-5G each
EUR 405.6

Gentamicin (sulfate)

HY-A0276 500mg
EUR 64.94
Description: Gentamicin sulfate, an orally active aminoglycoside antibiotic, inhibits the growth of both gram-positive and gram-negative bacteria and to inhibit several strains of mycoplasma in tissue culture. Gentamicin sulfate inhibits DNase I with an IC50 of 0.57 mM[1][2][3][4].

Gentamicin sulfate

GA7939 1g
EUR 115.8

Gentamicin sulfate

GA7939-1 1
EUR 15.9

Gentamicin sulfate

GA7939-10 10
EUR 71.1

Gentamicin sulfate

GA7939-10G 10 g
EUR 122.4

Gentamicin sulfate

GA7939-1G 1 g
EUR 55.2

Gentamicin sulfate

GA7939-25 25
EUR 126.5

Gentamicin sulfate

GA7939-25G 25 g
EUR 189.6

Gentamicin sulfate

GA7939-5 5
EUR 47.4

Gentamicin sulfate

GA7939-5G 5 g
EUR 93.6

GENTAMICIN SULFATE

G570 10G
EUR 32.75

Gentamicin sulfate

T1326-10mg 10mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-1g 1g Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-1mg 1mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-50mg 50mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-5mg 5mg Ask for price
Description: Gentamicin sulfate

Gentamicin X2 Sulfate

565235 1.0mg
EUR 380

Gentamicin X2 Sulfate

G360605 0.5mg
EUR 2223
Description: 57793-88-1

Gentamicin B Sulfate

G360650 25mg
EUR 25000
Description: 43169-50-2

Gentamicin Sulfate EP

G007-25G 25 g
EUR 316.27
Description: 1405-41-0

Gentamicin Sulfate EP

G007-5G 5 g
EUR 131.5
Description: 1405-41-0

Gentamicin Sulfate - 1g

PM-A4120/1 1g
EUR 5.89

Gentamicin Sulfate - 5g

PM-A4120/5 5g
EUR 18.7

Gentamicin Sulfate USP

G006-25G 25 g
EUR 220.51
Description: 1405-41-0

Gentamicin Sulfate USP

G006-5G 5 g
EUR 65.41
Description: 1405-41-0

Gentamycin (Gentamicin) Sulfate USP

MBS651447-100g 100g
EUR 570

Gentamycin (Gentamicin) Sulfate USP

MBS651447-1kg 1kg
EUR 1495

Gentamycin (Gentamicin) Sulfate USP

MBS651447-25g 25g
EUR 440

Gentamycin (Gentamicin) Sulfate USP

MBS651447-500g 500g
EUR 860

Gentamycin (Gentamicin) Sulfate USP

MBS651447-5g 5g
EUR 210

0.1g Gentamicin Sulfate Powder

61-098-RA EACH
EUR 58.37

1g Gentamicin Sulfate Powder

61-098-RF EACH
EUR 76.38

Gentamicin Sulfate, Animal-Free

19771-01 1G
EUR 53.2

Gentamicin Sulfate, Animal-Free

19771-14 5G
EUR 168

Gentamicin Sulfate, Animal-Free

19771-72 25G
EUR 630

Gentamicin Sulfate 10 mg/ml - 100ml

LM-A4111/100 100ml
EUR 20.19

Gentamicin Sulfate 50 mg/ml - 100ml

LM-A4112/100 100ml
EUR 30.25

Gentamicin C1 Sulfate EvoPure®

G031-10MG 10 mg
EUR 581.77
Description: 38539-12-7

Gentamicin C2 sulfate EvoPure®

G033-10MG 10 mg
EUR 581.77
Description: 25876-11-3 (free base)

Gentamicin A sulfate EvoPure®

G035-10MG 10 mg
EUR 318.2
Description: 13291-74-2

Gentamicin X2 sulfate EvoPure®

G036-2.5MG 2.5 mg Ask for price
Description: 36889-17-5

Gentamicin X2 sulfate EvoPure®

G036-PleaseContactusforPricing Please Contact us for Pricing Ask for price
Description: 36889-17-5

Gentamicin Sulfate 10 mg/ml - 10ml

LM-A4111/10 10ml
EUR 5.5

Gentamicin Sulfate 50 mg/ml - 10ml

LM-A4112/10 10ml
EUR 6.11

Gentamicin Sulfate Solution(50mg/ml)

11980-14 10ML
EUR 56

Gentamicin Sulfate Solution(10mg/ml)

16672-04 10ML
EUR 16.1

Gentamicin Sulfate Salt (C Complex) (Alcomicin, Bristagen, Cidomycin, Duragentam, Garamycin, Garasol,)

MBS6116877-25g 2.5(g
EUR 485

Gentamicin Sulfate Salt (C Complex) (Alcomicin, Bristagen, Cidomycin, Duragentam, Garamycin, Garasol,)

MBS6116877-5x25g 5x2.5g
EUR 2025

10mL Gentamicin Sulfate Liquid 5 - PK10

30-005-CR PK10
EUR 650.7

Gentamicin sulfate/Amphotericin (GA-1000)

LOCC-4083 each Ask for price

Gentamycin (Gentamicin) Sulfate Solution 10mg/ml

MBS651400-10x20mL 10x20mL
EUR 315

Gentamycin (Gentamicin) Sulfate Solution 10mg/ml

MBS651400-20mL 20mL
EUR 160

Gentamycin (Gentamicin) Sulfate Solution 10mg/ml

MBS651400-5x20mL 5x20mL
EUR 220

Gentamycin (Gentamicin) Sulfate Solution 50mg/ml

MBS651312-10x20mL 10x20mL
EUR 970

Gentamycin (Gentamicin) Sulfate Solution 50mg/ml

MBS651312-20mL 20mL
EUR 220

Gentamycin (Gentamicin) Sulfate Solution 50mg/ml

MBS651312-5x20mL 5x20mL
EUR 610

Gentamicin C1a sulfate EvoPure®

G032-10MG 10 mg
EUR 581.77
Description: 37713-04-5; 26098-04-4 (free base)

Gentamicin C2a sulfate EvoPure®

G034-10MG 10 mg
EUR 581.77
Description: 38539-12-7; 59751-72-3 (free base)

Gentamicin C2b sulfate EvoPure®

G062-10MG 10 mg
EUR 438.71
Description: 66803-19-8

Gentamicin Sulfate Solution, 50mg/mL, Sterile

40710024-1 20 mL
EUR 55.09

Gentamicin Sulfate Solution, 50mg/mL, Sterile

40710024-2 100 mL
EUR 137.56

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-1 20x1 mL
EUR 90.32

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-2 20 mL
EUR 66.21

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-3 50 mL
EUR 94.91

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-4 100 mL
EUR 142.53

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-5 100 mL
EUR 107.98

Gentamicin Sulfate Solution, 100mg/mL, Sterile

40120797-1 20 mL
EUR 72.55

Gentamicin Sulfate Solution, 100mg/mL, Sterile

40120797-2 100 mL
EUR 182.24
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