gentatest

modulates the gentamicin activity against multi-resistant strains and induces nefroprotection

by Krystal | Posted on

Dioclea violacea lectin modulates the gentamicin activity against multi-resistant strains and induces nefroprotection during antibiotic exposure.

Gentamicin is an aminoglycoside antibiotic used to treat infections of various origins. In the last few decades, the constant use of gentamicin has resulted in increased bacterial resistance and nephrotoxicity in some cases. In this study, we examined the ability of Dioclea violacea lectin (DVL) in modulate the antimicrobial activity of gentamicin and reduce the nephrotoxicity induced by this drug.

 

The minimum inhibitory concentration (MIC) obtained for DVL against all strains studied was not clinically relevant (MIC ≥ 1,024 μg/mL). However, when DVL was combined with gentamicin, a significant increase in antibiotic action was observed against Staphylococcus aureus and Escherichia coli. DVL also reduced antibiotic tolerance in S. aureus during 10 days of continuous treatment.

 

In addition, DVL presented a nephroprotective effect, reducing sodium excretion, N-Gal expression and urinary protein, that are important markers of glomerular and tubular injuries. Taken together, studies of inhibition of hemagglutinating activity, fluorescence spectroscopy and molecular docking revealed that gentamicin can interact with DVL via the carbohydrate recognition domain (CRD), suggesting that the results obtained in this study may be directly related to the interaction of DVL-gentamicin and with the ability of the lectin to interact with glycans present in the cells of the peritoneum.

Antioxidant, anti-inflammatory, and antiapoptotic effects of virgin coconut oil against antibiotic drug gentamicin-induced nephrotoxicity via the suppression of oxidative stress and modulation of iNOS/NF-ĸB/caspase-3 signaling pathway in Wistar rats.

Gentamicin is an effective antibiotic against severe infections; however, its major side effect is oxidative nephrotoxicity. We explored whether virgin coconut oil (VCO) could mitigate gentamicin-induced nephrotoxicity. Rats were fed with VCO-supplemented diet for 16 days against renal toxicity induced by gentamicin (100 mg/kg bw, ip) from Day 11 to 16. Gentamicin caused marked elevated serum urea, uric acid, and creatinine levels, followed by considerable depletion in renal antioxidant enzymes, glutathione (GSH), while the malondialdehyde (MDA) level increased significantly.

 

  • It significantly increased renal cytokines and nitric oxide (NO) levels, confirmed by renal histopathology. The expression of inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-ĸB), and caspase-3 was prominently increased.
  • VCO-supplemented diet significantly modulated the levels of biochemical indices, downregulated the expression of NO, iNOS, NF-ĸB, caspase-3, cytokines, and alleviated histopathological lesions. VCO protects against gentamicin-induced nephrotoxicity; thus, it could be a promising dietary supplement for patients undergoing gentamicin treatment.
  • PRACTICAL APPLICATIONS: Gentamicin is an efficacious clinical antibiotic used against severe infections; however, the robust body of evidence indicates that the nephrotoxic side effect constrained its use. Virgin coconut oil (VCO) is an edible oil with growing human consumption and pharmacological effects.
  • Our study has reported herein, for the first time, that VCO diet prevented the nephrotoxicity of gentamicin. Dietary supplementation of this oil could be beneficial in alleviating the nephrotoxic side effect of gentamicin in patients.
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Cotinine-BSA Conjugate
COTN15-BSA 0.5 mg
EUR 343.2

Bovine Serum Albumin Biotinylated (Biotin-BSA)
BTN-BSA 5 mg
EUR 343.2

Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate
IBT15-BSA 100 ug
EUR 634.8

Dinitrophenyl (DNP)-BSA protein Conjugate
AV-9330-BSA 10 mg
EUR 343.2

mPEG-BSA (Molecular Weight: 5,000-linear)
PEG-BSA-05K 100 ug
EUR 343.2

mPEG-BSA (Molecular Weight: 10,000-linear)
PEG-BSA-10K 100 ug
EUR 343.2

mPEG-BSA (Molecular Weight: 20,000-linear)
PEG-BSA-20K 100 ug
EUR 343.2

mPEG-BSA (Molecular Weight: 40,000-linear)
PEG-BSA-40K 100 ug
EUR 343.2

Gentamicin-HRP
65-IG10 1 mg
EUR 716.4
Description: Conjugated Gentamicin-HRP hapten

Gentamicin antibody
10-1541 100 ug
EUR 470.4
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody
10-G02A 500 ug
EUR 591.6
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody
20C-CR1031S 1 ml
EUR 262.8
Description: Sheep polyclonal Gentamicin antiserum

Gentamicin antibody
20-GG15 250 ul
EUR 220.8
Description: Goat polyclonal Gentamicin antibody

Gentamicin antibody
20-GR15 250 ul
EUR 249.6
Description: Rabbit polyclonal Gentamicin antibody

Gentamicin Antibody
abx021011-200ug 200 ug
EUR 393.6

Gentamicin Antibody
abx021012-1mg 1 mg
EUR 1144.8

Gentamicin Antibody
abx021013-1mg 1 mg
EUR 577.2

Gentamicin Antibody
20-abx210193
  • EUR 526.80
  • EUR 393.60
  • 100 ug
  • 50 ug

Gentamicin-OVA
80-1455 1 mg
EUR 781.2
Description: OVA conjugated Gentamicin Hapten

Gentamicin-OVA
80-1456 1 mg
EUR 781.2
Description: OVA conjugated Gentamicin Hapten

Gentamicin(GM)
AG022 1 mg
EUR 627.6

Gentamicin sulfate
B1270-1G
EUR 170.4

Gentamicin sulfate
B1270-25G
EUR 1136.4

Gentamicin sulfate
B1270-5G
EUR 405.6

Gentamicin(GM)
AT022 1mg
EUR 1134

Gentamicin [KLH]
DAG4484 1mg
EUR 1248

Gentamicin [HRP]
DAGA-032H 1mg
EUR 1248

Gentamicin (sulfate)
HY-A0276 500mg
EUR 142.8

Gentamicin sulfate
GA7939-10G 10 g
EUR 122.4

Gentamicin sulfate
GA7939-1G 1 g
EUR 55.2

Gentamicin sulfate
GA7939-25G 25 g
EUR 189.6

Gentamicin sulfate
GA7939-5G 5 g
EUR 93.6

(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA
DRAA31-BSA 0.5 mg
EUR 634.8

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA
PPPP321-BSA 0.5 mg
EUR 634.8

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA
NVDP41-BSA 0.5 mg
EUR 634.8

Gentamicin Solution, Gentamicin Solution, 50 mg/mL
CCM1123-010 10 mL
EUR 76.86

(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA
NANP51-BSA 0.5 mg
EUR 634.8

Bovine Serum Albumin (BSA) ELISA Kit
DLR-BSA-Ge-48T 48T
EUR 464.4
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) ELISA Kit
DLR-BSA-Ge-96T 96T
EUR 592.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column
800-302-BSA 1 Kit
EUR 416.4

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column
800-310-BSA 1 Kit
EUR 1074

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column
800-325-BSA 1 Kit
EUR 1740

General Bovine Serum Albumin (BSA) ELISA Kit
RD-BSA-Ge-48Tests 48 Tests
EUR 632.4

General Bovine Serum Albumin (BSA) ELISA Kit
RD-BSA-Ge-96Tests 96 Tests
EUR 878.4

General Bovine Serum Albumin (BSA) ELISA Kit
RDR-BSA-Ge-48Tests 48 Tests
EUR 661.2

General Bovine Serum Albumin (BSA) ELISA Kit
RDR-BSA-Ge-96Tests 96 Tests
EUR 919.2

Gentamicin ELISA kit
55R-2233 1 kit
EUR 837.6
Description: Gentamicin ELISA kit for use in research laboratory

Gentamicin monoclonal antibody
10R-11497 1 mg
EUR 1332
Description: Mouse anti- Gentamicin monoclonal antibody

Gentamicin sulfate salt
20-abx082235
  • EUR 226.80
  • EUR 343.20
  • 100 mg
  • 1 g

Gentamicin (GTM) Antibody
20-abx175177
  • EUR 393.60
  • EUR 994.80
  • EUR 526.80
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gentamicin ELISA Kit
DEIA047 96T
EUR 1238.4
Description: This kit can be used in quantitative and qualitative analysis of gentamicin residue in vaccine and cell culture.

Gentamicin, 50mg/ml
CA003-001 10ml
EUR 109.2

Gentamicin Sulfate USP
G006-25G 25 g
EUR 470.4

Gentamicin Sulfate USP
G006-5G 5 g
EUR 199.2

Gentamicin Sulfate EP
G007-25G 25 g
EUR 478.8

Gentamicin Sulfate EP
G007-5G 5 g
EUR 219.6

Gentamicin ELISA Kit
K4206-100
EUR 1188

Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant
AV-1010-BSA 1 ml
EUR 343.2

Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit
BCA-BSA-1 1 pk
EUR 196.8

Gentamicin ELISA Kit (OKAO00127)
OKAO00127 96 Wells
EUR 638.4
Description: Description of target: Gentamicin is a complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.;Species reactivity: General;Application: ;Assay info: Assay Methodology: Competitive Inhibition ELISA;Sensitivity: _x000D__x000D__x000D__x000D_
ComponentAmount
Tissue6 ppb
Liver10 ppb
Milk15 ppb
Serum6 ppb

Gentamicin C1 pentaacetate salt
GA1270-10MG 10 mg
EUR 783.6

Gentamicin C1 pentaacetate salt
GA1270-1MG 1 mg
EUR 204

Gentamicin C2 pentaacetate salt
GA2476-5MG 5 mg
EUR 418.8

Gentamicin C1a pentaacetate salt
GA4559-10MG 10 mg
EUR 514.8

Gentamicin C1a pentaacetate salt
GA4559-25MG 25 mg
EUR 964.8

0.1g Gentamicin Sulfate Powder
61-098-RA EACH
EUR 58.37

1g Gentamicin Sulfate Powder
61-098-RF EACH
EUR 76.38

Sd Gentamicin Gm-10
231299 PK10
EUR 50.86

Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit
BCA-BSA-750 1 ml
EUR 270

BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit
BSA-BGG-1 1 pk
EUR 343.2

Pre-diluted BSA (bovine serum albumin) standards (250, 125, 62.5, 31.25, and 0.0 ug/ml) for BCA Optima Protein Assay kit
BCA-BSA-2 1 pk
EUR 196.8

Mouse Anti Gentamicin Monoclonal Antibody
DMABT-54913MG 1 mg
EUR 920.4

Mouse Anti Gentamicin Monoclonal Antibody
DMABT-54914MG 1 mg
EUR 920.4

Sheep Anti Gentamicin Polyclonal Antibody
DPBT-68266SG 0.5 ml
EUR 920.4

Gentamicin C1 Sulfate EvoPure®
G031-10MG 10 mg
EUR 804

Gentamicin C1a sulfate EvoPure®
G032-10MG 10 mg
EUR 804

Gentamicin C2 sulfate EvoPure®
G033-10MG 10 mg
EUR 804

Gentamicin C2a sulfate EvoPure®
G034-10MG 10 mg
EUR 804

 

Gentamicin, azithromycin and ceftriaxone in the treatment of gonorrhoea: the relationship between antibiotic MIC and clinical outcome.

To investigate the relationship between MIC and clinical outcome in a randomized controlled trial that compared gentamicin 240 mg plus azithromycin 1 g with ceftriaxone 500 mg plus azithromycin 1 g. MIC analysis was performed on Neisseria gonorrhoeae isolates from all participants who were culture positive before they received treatment.

Viable gonococcal cultures were available from 279 participants, of whom 145 received ceftriaxone/azithromycin and 134 received gentamicin/azithromycin. Four participants (6 isolates) and 14 participants (17 isolates) did not clear infection in the ceftriaxone/azithromycin and gentamicin/azithromycin arms, respectively. MICs were determined by Etest on GC agar base with 1% Vitox.

 

The geometric mean MICs of azithromycin, ceftriaxone and gentamicin were compared using logistic and linear regression according to treatment received and N. gonorrhoeae clearance.As the azithromycin MIC increased, gentamicin/azithromycin treatment was less effective than ceftriaxone/azithromycin at clearing N. gonorrhoeae.

 

There was a higher geometric mean MIC of azithromycin for isolates from participants who had received gentamicin/azithromycin and did not clear infection compared with those who did clear infection [ratio 1.95 (95% CI 1.28-2.97)], but the use of categorical MIC breakpoints did not accurately predict the treatment response.

 

The geometric mean MIC of azithromycin was higher in isolates from the pharynx compared with genital isolates.We found that categorical resistance to azithromycin or ceftriaxone in vitro, and higher gentamicin MICs in the absence of breakpoints, were poorly predictive of treatment failure.

 

 

Gentamicin sulfate
GA7939-1G 1 g
EUR 55.2

Gentamicin sulfate
GA7939-25G 25 g
EUR 189.6

Gentamicin sulfate
GA7939-5G 5 g
EUR 93.6

Gentamicin Sulfate USP
G006-25G 25 g
EUR 470.4

Gentamicin Sulfate USP
G006-5G 5 g
EUR 199.2

Gentamicin Sulfate EP
G007-25G 25 g
EUR 478.8

Gentamicin Sulfate EP
G007-5G 5 g
EUR 219.6

Gentamicin C1 pentaacetate salt
GA1270-10MG 10 mg
EUR 783.6

Gentamicin C1 pentaacetate salt
GA1270-1MG 1 mg
EUR 204

Gentamicin C2 pentaacetate salt
GA2476-5MG 5 mg
EUR 418.8

Gentamicin C1a pentaacetate salt
GA4559-10MG 10 mg
EUR 514.8

Gentamicin C1a pentaacetate salt
GA4559-25MG 25 mg
EUR 964.8

0.1g Gentamicin Sulfate Powder
61-098-RA EACH
EUR 58.37

1g Gentamicin Sulfate Powder
61-098-RF EACH
EUR 76.38

Gentamicin C1 Sulfate EvoPure®
G031-10MG 10 mg
EUR 804

Gentamicin C1a sulfate EvoPure®
G032-10MG 10 mg
EUR 804

Gentamicin C2 sulfate EvoPure®
G033-10MG 10 mg
EUR 804

Gentamicin C2a sulfate EvoPure®
G034-10MG 10 mg
EUR 804

Gentamicin A sulfate EvoPure®
G035-10MG 10 mg
EUR 456

Gentamicin X2 sulfate EvoPure®
G036-2.5MG 2.5 mg
EUR 36

Gentamicin X2 sulfate EvoPure®
G036-PleaseContactusforPricing Please Contact us for Pricing
EUR 36

Gentamicin C2b sulfate EvoPure®
G062-10MG 10 mg
EUR 615.6

10mL Gentamicin Sulfate Liquid 5
30-005-CR PK10
EUR 523.26

CYT387 sulfate salt
A3339-10 10 mg
EUR 212.4
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

CYT387 sulfate salt
A3339-200 200 mg
EUR 1131.6
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

CYT387 sulfate salt
A3339-5 5 mg
EUR 170.4
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

CYT387 sulfate salt
A3339-50 50 mg
EUR 477.6
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

Streptomycin sulfate salt
20-abx082471
  • EUR 260.40
  • EUR 210.00
  • 25 g
  • 5 g

Streptomycin sulfate salt
20-abx082589
  • EUR 260.40
  • EUR 210.00
  • 25 g
  • 5 g

Netilmicin sulfate salt
GA1999-100MG 100 mg
EUR 74.4

Netilmicin sulfate salt
GA1999-1G 1 g
EUR 187.2

Netilmicin sulfate salt
GA1999-250MG 250 mg
EUR 103.2

Paromomycin sulfate salt
GA4947-1G 1 g
EUR 64.8

Paromomycin sulfate salt
GA4947-25G 25 g
EUR 274.8

Paromomycin sulfate salt
GA4947-5G 5 g
EUR 112.8

Streptomycin sulfate salt
GA7700-100G 100 g
EUR 93.6

Streptomycin sulfate salt
GA7700-25G 25 g
EUR 60

Streptomycin sulfate salt
GA7700-50G 50 g
EUR 74.4

Streptomycin sulfate salt
GA7700-5G 5 g
EUR 52.8

Ribostamycin sulfate salt
GA7826-1G 1 g
EUR 274.8

Ribostamycin sulfate salt
GA7826-250MG 250 mg
EUR 132

Sisomicin sulfate salt
GA7855-1G 1 g
EUR 170.4

Sisomicin sulfate salt
GA7855-250MG 250 mg
EUR 93.6

Sisomicin sulfate salt
GA7855-5G 5 g
EUR 409.2

Apramycin sulfate salt
GA8829-1G 1 g
EUR 60

Apramycin sulfate salt
GA8829-25G 25 g
EUR 141.6

Apramycin sulfate salt
GA8829-5G 5 g
EUR 74.4

Colistin sulfate salt
GA9867-100MG 100 mg
EUR 64.8

Colistin sulfate salt
GA9867-1G 1 g
EUR 132

Colistin sulfate salt
GA9867-500MG 500 mg
EUR 93.6

Colistin sulfate salt
GA9867-5G 5 g
EUR 370.8

Minoxidil sulfate salt
GP8215-100MG 100 mg
EUR 93.6

Minoxidil sulfate salt
GP8215-1G 1 g
EUR 163.2

Dodecyl sulfate sodium salt
abx185405-500g 500 g
EUR 427.2

Dextran sulfate sodium salt
B8205-100000 100 g
EUR 616.8

Dextran sulfate sodium salt
B8205-500000 500 g
EUR 1730.4

Polymixin B sulfate salt
GA4910-10G 10 g
EUR 322.8

Polymixin B sulfate salt
GA4910-1G 1 g
EUR 103.2

Polymixin B sulfate salt
GA4910-5G 5 g
EUR 208.8

Gentamicin Sulfate Solution (50 mg/mL in Water)
G046-20ML 20 mL
EUR 132

DHEA 3 Sulfate Sodium Salt
30-AD25 1 gram
EUR 412.8
Description: DHEA 3 Sulfate Sodium Salt

Chondroitin sulfate A sodium salt
GC6763-100G 100 g
EUR 237.6

Chondroitin sulfate A sodium salt
GC6763-10G 10 g
EUR 69.6

Chondroitin sulfate A sodium salt
GC6763-25G 25 g
EUR 102

Chondroitin sulfate A sodium salt
GC6763-50G 50 g
EUR 151.2

Chondroitin sulfate A sodium salt
GC6763-5G 5 g
EUR 60

Ethyl sulfate, sodium salt, 10 mg
0902-10 10 mg
EUR 158.4

Ethyl sulfate, sodium salt, 2 mg
0902-2 2 mg
EUR 82.8

Dextran Sulfate Sodium Salt, MW 8,000
D0800-025 25g
EUR 278.4

Dextran Sulfate Sodium Salt, MW 8,000
D0800-100 100g
EUR 619.2

Dextran Sulfate Sodium Salt, MW 8,000
D0800-101 1Kg
EUR 3705.6

Dextran Sulfate Sodium Salt, MW 500,000
D0811-010 10g
EUR 198

Dextran Sulfate Sodium Salt, MW 500,000
D0811-050 50g
EUR 273.6

Dextran Sulfate Sodium Salt, MW 500,000
D0811-100 100g
EUR 400.8

Dextran Sulfate Sodium Salt, MW 500,000
D0811-101 1Kg
EUR 3057.6

D-Glucosamine sulfate, potassium chloride salt
GC1036-100G 100 g
EUR 103.2

D-Glucosamine sulfate, potassium chloride salt
GC1036-250G 250 g
EUR 160.8

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