gentatest

modulates the gentamicin activity against multi-resistant strains and induces nefroprotection

Dioclea violacea lectin modulates the gentamicin activity against multi-resistant strains and induces nefroprotection during antibiotic exposure.

Gentamicin is an aminoglycoside antibiotic used to treat infections of various origins. In the last few decades, the constant use of gentamicin has resulted in increased bacterial resistance and nephrotoxicity in some cases. In this study, we examined the ability of Dioclea violacea lectin (DVL) in modulate the antimicrobial activity of gentamicin and reduce the nephrotoxicity induced by this drug.

 

The minimum inhibitory concentration (MIC) obtained for DVL against all strains studied was not clinically relevant (MIC ≥ 1,024 μg/mL). However, when DVL was combined with gentamicin, a significant increase in antibiotic action was observed against Staphylococcus aureus and Escherichia coli. DVL also reduced antibiotic tolerance in S. aureus during 10 days of continuous treatment.

 

In addition, DVL presented a nephroprotective effect, reducing sodium excretion, N-Gal expression and urinary protein, that are important markers of glomerular and tubular injuries. Taken together, studies of inhibition of hemagglutinating activity, fluorescence spectroscopy and molecular docking revealed that gentamicin can interact with DVL via the carbohydrate recognition domain (CRD), suggesting that the results obtained in this study may be directly related to the interaction of DVL-gentamicin and with the ability of the lectin to interact with glycans present in the cells of the peritoneum.

Antioxidant, anti-inflammatory, and antiapoptotic effects of virgin coconut oil against antibiotic drug gentamicin-induced nephrotoxicity via the suppression of oxidative stress and modulation of iNOS/NF-ĸB/caspase-3 signaling pathway in Wistar rats.

Gentamicin is an effective antibiotic against severe infections; however, its major side effect is oxidative nephrotoxicity. We explored whether virgin coconut oil (VCO) could mitigate gentamicin-induced nephrotoxicity. Rats were fed with VCO-supplemented diet for 16 days against renal toxicity induced by gentamicin (100 mg/kg bw, ip) from Day 11 to 16. Gentamicin caused marked elevated serum urea, uric acid, and creatinine levels, followed by considerable depletion in renal antioxidant enzymes, glutathione (GSH), while the malondialdehyde (MDA) level increased significantly.

 

  • It significantly increased renal cytokines and nitric oxide (NO) levels, confirmed by renal histopathology. The expression of inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-ĸB), and caspase-3 was prominently increased.
  • VCO-supplemented diet significantly modulated the levels of biochemical indices, downregulated the expression of NO, iNOS, NF-ĸB, caspase-3, cytokines, and alleviated histopathological lesions. VCO protects against gentamicin-induced nephrotoxicity; thus, it could be a promising dietary supplement for patients undergoing gentamicin treatment.
  • PRACTICAL APPLICATIONS: Gentamicin is an efficacious clinical antibiotic used against severe infections; however, the robust body of evidence indicates that the nephrotoxic side effect constrained its use. Virgin coconut oil (VCO) is an edible oil with growing human consumption and pharmacological effects.
  • Our study has reported herein, for the first time, that VCO diet prevented the nephrotoxicity of gentamicin. Dietary supplementation of this oil could be beneficial in alleviating the nephrotoxic side effect of gentamicin in patients.
gentatest
gentatest

 

Gentamicin-BSA

80-IG10 25 mg
EUR 381
Description: Conjugated Gentamicin-BSA hapten

BSA Control for Age-BSA

2221-BSA
EUR 175

Cotinine-BSA Conjugate

COTN15-BSA 0.5 mg
EUR 286

Bovine Serum Albumin Biotinylated (Biotin-BSA)

BTN-BSA 5 mg
EUR 286

Ibrutinib drug-Bovine Serum Albumin (BSA) Conjugate

IBT15-BSA 100 ug
EUR 529

Dinitrophenyl (DNP)-BSA protein Conjugate

AV-9330-BSA 10 mg
EUR 286

mPEG-BSA (Molecular Weight: 5,000-linear)

PEG-BSA-05K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 10,000-linear)

PEG-BSA-10K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 20,000-linear)

PEG-BSA-20K 100 ug
EUR 286

mPEG-BSA (Molecular Weight: 40,000-linear)

PEG-BSA-40K 100 ug
EUR 286

Gentamicin antibody

20C-CR1031S 1 ml
EUR 219
Description: Sheep polyclonal Gentamicin antiserum

Gentamicin antibody

10-1541 100 ug
EUR 392
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody

10-G02A 500 ug
EUR 493
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody

20-GG15 250 ul
EUR 184
Description: Goat polyclonal Gentamicin antibody

Gentamicin antibody

20-GR15 250 ul
EUR 208
Description: Rabbit polyclonal Gentamicin antibody

Gentamicin-HRP

65-IG10 1 mg
EUR 597
Description: Conjugated Gentamicin-HRP hapten

Gentamicin sulfate

B1270-1G
EUR 142

Gentamicin sulfate

B1270-25G
EUR 947

Gentamicin sulfate

B1270-5G
EUR 338

Gentamicin(GM)

AT022 1mg
EUR 945

Gentamicin [KLH]

DAG4484 1mg
EUR 1040

Gentamicin [HRP]

DAGA-032H 1mg
EUR 1040

Gentamicin-OVA

80-1455 1 mg
EUR 651
Description: OVA conjugated Gentamicin Hapten

Gentamicin-OVA

80-1456 1 mg
EUR 651
Description: OVA conjugated Gentamicin Hapten

Gentamicin Antibody

abx021011-200ug 200 ug
EUR 328
  • Shipped within 5-10 working days.

Gentamicin Antibody

abx021012-1mg 1 mg
EUR 954
  • Shipped within 5-10 working days.

Gentamicin Antibody

abx021013-1mg 1 mg
EUR 481
  • Shipped within 5-10 working days.

Gentamicin Antibody

20-abx210193
  • EUR 439.00
  • EUR 328.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.

Gentamicin(GM)

AG022 1 mg
EUR 523

Gentamicin (sulfate)

HY-A0276 500mg
EUR 119

Gentamicin sulfate

GA7939-10G 10 g
EUR 102

Gentamicin sulfate

GA7939-1G 1 g
EUR 46

Gentamicin sulfate

GA7939-25G 25 g
EUR 158

Gentamicin sulfate

GA7939-5G 5 g
EUR 78

(DRAAGQPAG)3 peptide (repeat-sequence peptide of the P. vivax circumsporozoite protein, CSP) conjugated with BSA

DRAA31-BSA 0.5 mg
EUR 529

(PPPPNAND)3 peptide (repeat-sequence peptide of the P. berghei circumsporozoite protein, CSP) conjugated with BSA

PPPP321-BSA 0.5 mg
EUR 529

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 64.05
  • Product category: Culture Media

(NVDP)4 peptide (minor repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NVDP41-BSA 0.5 mg
EUR 529

(NANP)5 peptide (25-aa, repeat-sequence peptide of the P. falciparum circumsporozoite protein, CSP) conjugated with BSA

NANP51-BSA 0.5 mg
EUR 529

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-48T 48T
EUR 387
  • Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Bovine Serum Albumin (BSA) ELISA Kit

DLR-BSA-Ge-96T 96T
EUR 494
  • Should the Bovine Serum Albumin (BSA) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A competitive inhibition quantitative ELISA assay kit for detection of Bovine Serum Albumin (BSA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Gentamicin monoclonal antibody

10R-11497 1 mg
EUR 1110
Description: Mouse anti- Gentamicin monoclonal antibody

Gentamicin ELISA kit

55R-2233 1 kit
EUR 698
Description: Gentamicin ELISA kit for use in research laboratory

Gentamicin ELISA Kit

DEIA047 96T
EUR 1032
Description: This kit can be used in quantitative and qualitative analysis of gentamicin residue in vaccine and cell culture.

Gentamicin (GTM) Antibody

20-abx175177
  • EUR 328.00
  • EUR 829.00
  • EUR 439.00
  • EUR 154.00
  • EUR 258.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug
  • Shipped within 5-7 working days.

Gentamicin sulfate salt

20-abx082235
  • EUR 189.00
  • EUR 286.00
  • 100 mg
  • 1 g
  • Shipped within 5-10 working days.

Gentamicin, 50mg/ml

CA003-001 10ml
EUR 91

Gentamicin Sulfate USP

G006-25G 25 g
EUR 392

Gentamicin Sulfate USP

G006-5G 5 g
EUR 166

Gentamicin Sulfate EP

G007-25G 25 g
EUR 399

Gentamicin Sulfate EP

G007-5G 5 g
EUR 183

Gentamicin ELISA Kit

K4206-100
EUR 990

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 1-2 mg BSA from Bioprocessed material), 2 ml aff column

800-302-BSA 1 Kit
EUR 347

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 10-20 mg BSA from Bioprocessed material), 10 ml aff column

800-310-BSA 1 Kit
EUR 895

Bovine serum albumin (BSA) removal kit (Antibody based aff matrix; sufficient to remove 25-50 mg BSA from Bioprocessed material), 25 ml aff column

800-325-BSA 1 Kit
EUR 1450

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-48Tests 48 Tests
EUR 551

General Bovine Serum Albumin (BSA) ELISA Kit

RDR-BSA-Ge-96Tests 96 Tests
EUR 766

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-48Tests 48 Tests
EUR 527

General Bovine Serum Albumin (BSA) ELISA Kit

RD-BSA-Ge-96Tests 96 Tests
EUR 732

Vaccigel Alum adjuvant adsorbed with Bovine Serum Albumin (BSA @1 mg/ml), Vaccine adjuvant

AV-1010-BSA 1 ml
EUR 286

Gentamicin C1 pentaacetate salt

GA1270-10MG 10 mg
EUR 653

Gentamicin C1 pentaacetate salt

GA1270-1MG 1 mg
EUR 170

Gentamicin C2 pentaacetate salt

GA2476-5MG 5 mg
EUR 349

Gentamicin C1a pentaacetate salt

GA4559-10MG 10 mg
EUR 429

Gentamicin C1a pentaacetate salt

GA4559-25MG 25 mg
EUR 804

Gentamicin ELISA Kit (OKAO00127)

OKAO00127 96 Wells
EUR 532
Description: Description of target: Gentamicin is a complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.;Species reactivity: General;Application: ;Assay info: Assay Methodology: Competitive Inhibition ELISA;Sensitivity: _x000D__x000D__x000D__x000D_
ComponentAmount
Tissue6 ppb
Liver10 ppb
Milk15 ppb
Serum6 ppb

Pre-diluted BSA (bovine serum albumin) standards (1 set) for BCA Optima Protein Assay kit

BCA-BSA-1 1 pk
EUR 164

Pre-diluted BSA (bovine serum albumin) standards (750 ug/ml) for BCA Optima Protein Assay kit

BCA-BSA-750 1 ml
EUR 225

BSA and BGG Stock standards (1 vial each @ 2 mg/ml) for BCA Optima Protein Assay kit

BSA-BGG-1 1 pk
EUR 286

0.1 G GENTAMICIN SULFATE, POWDER

61-098-RA 0,1 g/pk
EUR 62
Description: Media Catalog; Cell Culture Reagents

1 G GENTAMICIN SULFATE, POWDER

61-098-RF 1 g/pk
EUR 58
Description: Media Catalog; Cell Culture Reagents

Mouse Anti Gentamicin Monoclonal Antibody

DMABT-54913MG 1 mg
EUR 767

Mouse Anti Gentamicin Monoclonal Antibody

DMABT-54914MG 1 mg
EUR 767

Sheep Anti Gentamicin Polyclonal Antibody

DPBT-68266SG 0.5 ml
EUR 767

Gentamicin C1 Sulfate EvoPure®

G031-10MG 10 mg
EUR 670

Gentamicin C1a sulfate EvoPure®

G032-10MG 10 mg
EUR 670

Gentamicin C2 sulfate EvoPure®

G033-10MG 10 mg
EUR 670

Gentamicin C2a sulfate EvoPure®

G034-10MG 10 mg
EUR 670

 

Gentamicin, azithromycin and ceftriaxone in the treatment of gonorrhoea: the relationship between antibiotic MIC and clinical outcome.

To investigate the relationship between MIC and clinical outcome in a randomized controlled trial that compared gentamicin 240 mg plus azithromycin 1 g with ceftriaxone 500 mg plus azithromycin 1 g. MIC analysis was performed on Neisseria gonorrhoeae isolates from all participants who were culture positive before they received treatment.

Viable gonococcal cultures were available from 279 participants, of whom 145 received ceftriaxone/azithromycin and 134 received gentamicin/azithromycin. Four participants (6 isolates) and 14 participants (17 isolates) did not clear infection in the ceftriaxone/azithromycin and gentamicin/azithromycin arms, respectively. MICs were determined by Etest on GC agar base with 1% Vitox.

 

The geometric mean MICs of azithromycin, ceftriaxone and gentamicin were compared using logistic and linear regression according to treatment received and N. gonorrhoeae clearance.As the azithromycin MIC increased, gentamicin/azithromycin treatment was less effective than ceftriaxone/azithromycin at clearing N. gonorrhoeae.

 

There was a higher geometric mean MIC of azithromycin for isolates from participants who had received gentamicin/azithromycin and did not clear infection compared with those who did clear infection [ratio 1.95 (95% CI 1.28-2.97)], but the use of categorical MIC breakpoints did not accurately predict the treatment response.

 

The geometric mean MIC of azithromycin was higher in isolates from the pharynx compared with genital isolates.We found that categorical resistance to azithromycin or ceftriaxone in vitro, and higher gentamicin MICs in the absence of breakpoints, were poorly predictive of treatment failure.

 

 

Gentamicin sulfate

B1270-5G
EUR 338

Gentamicin (sulfate)

HY-A0276 500mg
EUR 119

Gentamicin sulfate

GA7939-10G 10 g
EUR 102

Gentamicin sulfate

GA7939-1G 1 g
EUR 46

Gentamicin sulfate

GA7939-25G 25 g
EUR 158

Gentamicin sulfate

GA7939-5G 5 g
EUR 78

Gentamicin Sulfate USP

G006-25G 25 g
EUR 392

Gentamicin Sulfate USP

G006-5G 5 g
EUR 166

Gentamicin Sulfate EP

G007-25G 25 g
EUR 399

Gentamicin Sulfate EP

G007-5G 5 g
EUR 183

Gentamicin C1 pentaacetate salt

GA1270-10MG 10 mg
EUR 653

Gentamicin C1 pentaacetate salt

GA1270-1MG 1 mg
EUR 170

Gentamicin C2 pentaacetate salt

GA2476-5MG 5 mg
EUR 349

Gentamicin C1a pentaacetate salt

GA4559-10MG 10 mg
EUR 429

Gentamicin C1a pentaacetate salt

GA4559-25MG 25 mg
EUR 804

0.1 G GENTAMICIN SULFATE, POWDER

61-098-RA 0,1 g/pk
EUR 62
Description: Media Catalog; Cell Culture Reagents

1 G GENTAMICIN SULFATE, POWDER

61-098-RF 1 g/pk
EUR 58
Description: Media Catalog; Cell Culture Reagents

Gentamicin C1 Sulfate EvoPure®

G031-10MG 10 mg
EUR 670

Gentamicin C1a sulfate EvoPure®

G032-10MG 10 mg
EUR 670

Gentamicin C2 sulfate EvoPure®

G033-10MG 10 mg
EUR 670

Gentamicin C2a sulfate EvoPure®

G034-10MG 10 mg
EUR 670

Gentamicin A sulfate EvoPure®

G035-10MG 10 mg
EUR 380

Gentamicin X2 sulfate EvoPure®

G036-2.5MG 2.5 mg
EUR 30

Gentamicin X2 sulfate EvoPure®

G036-PleaseContactusforPricing Please Contact us for Pricing
EUR 30

Gentamicin C2b sulfate EvoPure®

G062-10MG 10 mg
EUR 513

Streptomycin sulfate salt

20-abx082471
  • EUR 217.00
  • EUR 175.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

Streptomycin sulfate salt

20-abx082589
  • EUR 217.00
  • EUR 175.00
  • 25 g
  • 5 g
  • Shipped within 5-10 working days.

CYT387 sulfate salt

A3339-10 10 mg
EUR 177
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

CYT387 sulfate salt

A3339-200 200 mg
EUR 943
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

CYT387 sulfate salt

A3339-5 5 mg
EUR 142
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

CYT387 sulfate salt

A3339-50 50 mg
EUR 398
Description: CYT387 is an ATP-competitive small molecule JAK1/JAK2 inhibitor with IC50 of 11 and 18 nM for JAK1 and JAK2, respectively. CYT387 is useful for treatment of myeloproliferative disorders and anti-cancer.

Minoxidil sulfate salt

GP8215-100MG 100 mg
EUR 78

Minoxidil sulfate salt

GP8215-1G 1 g
EUR 136

Netilmicin sulfate salt

GA1999-100MG 100 mg
EUR 62

Netilmicin sulfate salt

GA1999-1G 1 g
EUR 156

Netilmicin sulfate salt

GA1999-250MG 250 mg
EUR 86

Paromomycin sulfate salt

GA4947-1G 1 g
EUR 54

Paromomycin sulfate salt

GA4947-25G 25 g
EUR 229

Paromomycin sulfate salt

GA4947-5G 5 g
EUR 94

Streptomycin sulfate salt

GA7700-100G 100 g
EUR 78

Streptomycin sulfate salt

GA7700-25G 25 g
EUR 50

Streptomycin sulfate salt

GA7700-50G 50 g
EUR 62

Streptomycin sulfate salt

GA7700-5G 5 g
EUR 44

Ribostamycin sulfate salt

GA7826-1G 1 g
EUR 229

Ribostamycin sulfate salt

GA7826-250MG 250 mg
EUR 110

Sisomicin sulfate salt

GA7855-1G 1 g
EUR 142

Sisomicin sulfate salt

GA7855-250MG 250 mg
EUR 78

Sisomicin sulfate salt

GA7855-5G 5 g
EUR 341

Apramycin sulfate salt

GA8829-1G 1 g
EUR 50

Apramycin sulfate salt

GA8829-25G 25 g
EUR 118

Apramycin sulfate salt

GA8829-5G 5 g
EUR 62

Colistin sulfate salt

GA9867-100MG 100 mg
EUR 54

Colistin sulfate salt

GA9867-1G 1 g
EUR 110

Colistin sulfate salt

GA9867-500MG 500 mg
EUR 78

Colistin sulfate salt

GA9867-5G 5 g
EUR 309

Dodecyl sulfate sodium salt

abx185405-500g 500 g
EUR 356
  • Shipped within 1-2 weeks.

Dextran sulfate sodium salt

B8205-100000 100 g
EUR 514

Dextran sulfate sodium salt

B8205-500000 500 g
EUR 1442

Polymixin B sulfate salt

GA4910-10G 10 g
EUR 269

Polymixin B sulfate salt

GA4910-1G 1 g
EUR 86

Polymixin B sulfate salt

GA4910-5G 5 g
EUR 174

Gentamicin Sulfate Solution (50 mg/mL in Water)

G046-20ML 20 mL
EUR 110

DHEA 3 Sulfate Sodium Salt

30-AD25 1 gram
EUR 344
Description: DHEA 3 Sulfate Sodium Salt

Chondroitin sulfate A sodium salt

GC6763-100G 100 g
EUR 198

Chondroitin sulfate A sodium salt

GC6763-10G 10 g
EUR 58

Chondroitin sulfate A sodium salt

GC6763-25G 25 g
EUR 85

Chondroitin sulfate A sodium salt

GC6763-50G 50 g
EUR 126

Chondroitin sulfate A sodium salt

GC6763-5G 5 g
EUR 50

10 ML GENTAMICIN SULFATE, LIQUID 50 MG/ML SOLUTION

30-005-CR 10 mL/pk
EUR 433
Description: Media Catalog; Cell Culture Reagents

Ethyl sulfate, sodium salt, 10 mg

0902-10 10 mg
EUR 132

Ethyl sulfate, sodium salt, 2 mg

0902-2 2 mg
EUR 69

Dextran Sulfate Sodium Salt, MW 8,000

D0800-025 25g
EUR 232

Dextran Sulfate Sodium Salt, MW 8,000

D0800-100 100g
EUR 516

Dextran Sulfate Sodium Salt, MW 8,000

D0800-101 1Kg
EUR 3088

Dextran Sulfate Sodium Salt, MW 500,000

D0811-010 10g
EUR 165

Dextran Sulfate Sodium Salt, MW 500,000

D0811-050 50g
EUR 228

Dextran Sulfate Sodium Salt, MW 500,000

D0811-100 100g
EUR 334

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