gentatest

modulates the gentamicin activity against multi-resistant strains and induces nefroprotection

Dioclea violacea lectin modulates the gentamicin activity against multi-resistant strains and induces nefroprotection during antibiotic exposure.

Gentamicin is an aminoglycoside antibiotic used to treat infections of various origins. In the last few decades, the constant use of gentamicin has resulted in increased bacterial resistance and nephrotoxicity in some cases. In this study, we examined the ability of Dioclea violacea lectin (DVL) in modulate the antimicrobial activity of gentamicin and reduce the nephrotoxicity induced by this drug.

 

The minimum inhibitory concentration (MIC) obtained for DVL against all strains studied was not clinically relevant (MIC ≥ 1,024 μg/mL). However, when DVL was combined with gentamicin, a significant increase in antibiotic action was observed against Staphylococcus aureus and Escherichia coli. DVL also reduced antibiotic tolerance in S. aureus during 10 days of continuous treatment.

 

In addition, DVL presented a nephroprotective effect, reducing sodium excretion, N-Gal expression and urinary protein, that are important markers of glomerular and tubular injuries. Taken together, studies of inhibition of hemagglutinating activity, fluorescence spectroscopy and molecular docking revealed that gentamicin can interact with DVL via the carbohydrate recognition domain (CRD), suggesting that the results obtained in this study may be directly related to the interaction of DVL-gentamicin and with the ability of the lectin to interact with glycans present in the cells of the peritoneum.

Antioxidant, anti-inflammatory, and antiapoptotic effects of virgin coconut oil against antibiotic drug gentamicin-induced nephrotoxicity via the suppression of oxidative stress and modulation of iNOS/NF-ĸB/caspase-3 signaling pathway in Wistar rats.

Gentamicin is an effective antibiotic against severe infections; however, its major side effect is oxidative nephrotoxicity. We explored whether virgin coconut oil (VCO) could mitigate gentamicin-induced nephrotoxicity. Rats were fed with VCO-supplemented diet for 16 days against renal toxicity induced by gentamicin (100 mg/kg bw, ip) from Day 11 to 16. Gentamicin caused marked elevated serum urea, uric acid, and creatinine levels, followed by considerable depletion in renal antioxidant enzymes, glutathione (GSH), while the malondialdehyde (MDA) level increased significantly.

 

  • It significantly increased renal cytokines and nitric oxide (NO) levels, confirmed by renal histopathology. The expression of inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-ĸB), and caspase-3 was prominently increased.
  • VCO-supplemented diet significantly modulated the levels of biochemical indices, downregulated the expression of NO, iNOS, NF-ĸB, caspase-3, cytokines, and alleviated histopathological lesions. VCO protects against gentamicin-induced nephrotoxicity; thus, it could be a promising dietary supplement for patients undergoing gentamicin treatment.
  • PRACTICAL APPLICATIONS: Gentamicin is an efficacious clinical antibiotic used against severe infections; however, the robust body of evidence indicates that the nephrotoxic side effect constrained its use. Virgin coconut oil (VCO) is an edible oil with growing human consumption and pharmacological effects.
  • Our study has reported herein, for the first time, that VCO diet prevented the nephrotoxicity of gentamicin. Dietary supplementation of this oil could be beneficial in alleviating the nephrotoxic side effect of gentamicin in patients.
gentatest
gentatest

 

Gentamicin-BSA

80-IG10 25 mg
EUR 325
Description: Conjugated Gentamicin-BSA hapten

Gentamicin

G272 10ml
EUR 70

Gentamicin

MD025-1PK 1 unit Ask for price
Description: Gentamicin

Gentamicin

MD061-1PK 1 unit Ask for price
Description: Gentamicin

Gentamicin

EM025-150ST 1 unit
EUR 164.38
Description: Gentamicin

Gentamicin

EM061-150ST 1 unit
EUR 169.41
Description: Gentamicin

Gentamicin

T25447-10mg 10mg Ask for price
Description: Gentamicin

Gentamicin

T25447-1g 1g Ask for price
Description: Gentamicin

Gentamicin

T25447-1mg 1mg Ask for price
Description: Gentamicin

Gentamicin

T25447-50mg 50mg Ask for price
Description: Gentamicin

Gentamicin

T25447-5mg 5mg Ask for price
Description: Gentamicin

Gentamicin Solution, Gentamicin Solution, 50 mg/mL

CCM1123-010 10 mL
EUR 76.86

Gentamicin(GM)

AT022 1mg
EUR 1134

Gentamicin(GM)

AG022 1 mg
EUR 627.6

Gentamicin [KLH]

DAG4484 1mg
EUR 1248

Gentamicin [HRP]

DAGA-032H 1mg
EUR 1248

Gentamicin [OVA]

DAG4468O 1 mg
EUR 520
Description: Synthetic

Gentamicin-HRP

65-IG10 1 mg
EUR 525
Description: Conjugated Gentamicin-HRP hapten

Gentamicin-OVA

80-1455 1 mg
EUR 592
Description: OVA conjugated Gentamicin Hapten

Gentamicin-OVA

80-1456 1 mg
EUR 592
Description: OVA conjugated Gentamicin Hapten

Gentamicin Sulfate

08975-81 1G
EUR 77

Gentamicin Sulfate

08975-94 5G
EUR 266

Gentamicin Sulfate

16637-32 25G
EUR 630

Gentamicin sulfate

329895 1.0g
EUR 250

Gentamicin Sulfate

40710020-1 5 g
EUR 26.88

Gentamicin Sulfate

40710020-2 10 g
EUR 46.61

Gentamicin Sulfate

40710020-3 25 g
EUR 76.8

Gentamicin sulfate

B1270-1G each
EUR 170.4

Gentamicin sulfate

B1270-25G each
EUR 1136.4

Gentamicin sulfate

B1270-5G each
EUR 405.6

Gentamicin (sulfate)

HY-A0276 500mg
EUR 142.8

Gentamicin sulfate

GA7939-1 1
EUR 15.9

Gentamicin sulfate

GA7939-10 10
EUR 71.1

Gentamicin sulfate

GA7939-10G 10 g
EUR 122.4

Gentamicin sulfate

GA7939-1G 1 g
EUR 55.2

Gentamicin sulfate

GA7939-25 25
EUR 126.5

Gentamicin sulfate

GA7939-25G 25 g
EUR 189.6

Gentamicin sulfate

GA7939-5 5
EUR 47.4

Gentamicin sulfate

GA7939-5G 5 g
EUR 93.6

GENTAMICIN SULFATE

G570 10G
EUR 32.75

Gentamicin sulfate

T1326-10mg 10mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-1g 1g Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-1mg 1mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-50mg 50mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-5mg 5mg Ask for price
Description: Gentamicin sulfate

Gentamicin Antibody

abx021011-200ug 200 ug
EUR 393.6

Gentamicin Antibody

abx021012-1mg 1 mg
EUR 1144.8

Gentamicin Antibody

abx021013-1mg 1 mg
EUR 577.2

Gentamicin antibody

10-1541 100 ug
EUR 350
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody

10-G02A 500 ug
EUR 231.75
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody

20-GG15 250 ul Ask for price
Description: Goat polyclonal Gentamicin antibody

Gentamicin antibody

20-GR15 250 ul
EUR 165
Description: Rabbit polyclonal Gentamicin antibody

Gentamicin antibody

20C-CR1031S 1 ml
EUR 174
Description: Sheep polyclonal Gentamicin antiserum

Gentamicin Antibody

20-abx210193
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

Gentamicin Antibody

GWB-5E5C0A 1 ml Ask for price

Gentamicin Antibody

GWB-5F0BB2 1 mg Ask for price

Gentamicin Antibody

GWB-300476 0.2 mg Ask for price

Gentamicin Antibody

GWB-Q00939 1 mg Ask for price

Gentamicin Antibody

GWB-Q00940 1 mg Ask for price

Gentamicin, Antibody

GWB-F36A07 1 mg Ask for price

Gentamicin Antibody

GWB-C38937 0.2 mg Ask for price

Gentamicin sulphate

PCT1118-10G 1 unit
EUR 85.92
Description: Gentamicin sulphate

Gentamicin sulphate

PCT1118-25G 1 unit
EUR 212.21
Description: Gentamicin sulphate

Gentamicin sulphate

CMS461-1G 1 unit
EUR 8.67
Description: Gentamicin sulphate

Gentamicin sulphate

CMS461-5G 1 unit
EUR 39.01
Description: Gentamicin sulphate

Gentamicin Antibody

abx210193-100l 100 µl
EUR 400

Gentamicin Antibody

abx210193-50l 50 µl
EUR 281.25

Gentamicin Antibody

abx021012-400l 400 µl Ask for price

Gentamicin Antibody

abx021012-80l 80 µl
EUR 2012.5

Gentamicin, 50mg/ml

CA003-001 10ml
EUR 109.2

Gentamicin ELISA Kit

DEIA047 96T
EUR 1238.4
Description: This kit can be used in quantitative and qualitative analysis of gentamicin residue in vaccine and cell culture.

Gentamicin ELISA kit

55R-2233 1 kit
EUR 837.6
Description: Gentamicin ELISA kit for use in research laboratory

Gentamicin ELISA Kit

K4206-100 each
EUR 1188

Gentamicin ELISA Kit

E6208 96T
EUR 200
Description: milk, meat

Gentamicin X2 Sulfate

565235 1.0mg
EUR 380

Gentamicin Sulfate EP

G007-25G 25 g
EUR 309.86
Description: C21H43N5O7 • H2SO4

Gentamicin Sulfate EP

G007-5G 5 g
EUR 128.8
Description: C21H43N5O7 • H2SO4

Gentamicin X2 Sulfate

G360605 0.5mg
EUR 2223
Description: 57793-88-1

 

Gentamicin, azithromycin and ceftriaxone in the treatment of gonorrhoea: the relationship between antibiotic MIC and clinical outcome.

To investigate the relationship between MIC and clinical outcome in a randomized controlled trial that compared gentamicin 240 mg plus azithromycin 1 g with ceftriaxone 500 mg plus azithromycin 1 g. MIC analysis was performed on Neisseria gonorrhoeae isolates from all participants who were culture positive before they received treatment.

Viable gonococcal cultures were available from 279 participants, of whom 145 received ceftriaxone/azithromycin and 134 received gentamicin/azithromycin. Four participants (6 isolates) and 14 participants (17 isolates) did not clear infection in the ceftriaxone/azithromycin and gentamicin/azithromycin arms, respectively. MICs were determined by Etest on GC agar base with 1% Vitox.

 

The geometric mean MICs of azithromycin, ceftriaxone and gentamicin were compared using logistic and linear regression according to treatment received and N. gonorrhoeae clearance.As the azithromycin MIC increased, gentamicin/azithromycin treatment was less effective than ceftriaxone/azithromycin at clearing N. gonorrhoeae.

 

There was a higher geometric mean MIC of azithromycin for isolates from participants who had received gentamicin/azithromycin and did not clear infection compared with those who did clear infection [ratio 1.95 (95% CI 1.28-2.97)], but the use of categorical MIC breakpoints did not accurately predict the treatment response.

 

The geometric mean MIC of azithromycin was higher in isolates from the pharynx compared with genital isolates.We found that categorical resistance to azithromycin or ceftriaxone in vitro, and higher gentamicin MICs in the absence of breakpoints, were poorly predictive of treatment failure.

 

 

Gentamicin Sulfate

08975-94 5G
EUR 266

Gentamicin Sulfate

16637-32 25G
EUR 630

Gentamicin sulfate

329895 1.0g
EUR 250

Gentamicin Sulfate

40710020-1 5 g
EUR 26.88

Gentamicin Sulfate

40710020-2 10 g
EUR 46.61

Gentamicin Sulfate

40710020-3 25 g
EUR 76.8

GENTAMICIN SULFATE

G570 10G
EUR 32.75

Gentamicin sulfate

GA7939-1 1
EUR 15.9

Gentamicin sulfate

GA7939-10 10
EUR 71.1

Gentamicin sulfate

GA7939-10G 10 g
EUR 122.4

Gentamicin sulfate

GA7939-1G 1 g
EUR 55.2

Gentamicin sulfate

GA7939-25 25
EUR 126.5

Gentamicin sulfate

GA7939-25G 25 g
EUR 189.6

Gentamicin sulfate

GA7939-5 5
EUR 47.4

Gentamicin sulfate

GA7939-5G 5 g
EUR 93.6

Gentamicin sulfate

B1270-1G each
EUR 170.4

Gentamicin sulfate

B1270-25G each
EUR 1136.4

Gentamicin sulfate

B1270-5G each
EUR 405.6

Gentamicin (sulfate)

HY-A0276 500mg
EUR 142.8

Gentamicin sulfate

T1326-10mg 10mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-1g 1g Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-1mg 1mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-50mg 50mg Ask for price
Description: Gentamicin sulfate

Gentamicin sulfate

T1326-5mg 5mg Ask for price
Description: Gentamicin sulfate

Gentamicin Sulfate EP

G007-25G 25 g
EUR 309.86
Description: C21H43N5O7 • H2SO4

Gentamicin Sulfate EP

G007-5G 5 g
EUR 128.8
Description: C21H43N5O7 • H2SO4

Gentamicin X2 Sulfate

G360605 0.5mg
EUR 2223
Description: 57793-88-1

Gentamicin B Sulfate

G360650 25mg
EUR 25000
Description: 43169-50-2

Gentamicin X2 Sulfate

565235 1.0mg
EUR 380

Gentamicin Sulfate - 1g

PM-A4120/1 1g
EUR 5.89

Gentamicin Sulfate - 5g

PM-A4120/5 5g
EUR 18.7

Gentamicin Sulfate USP

G006-25G 25 g
EUR 215.79
Description: N/A

Gentamicin Sulfate USP

G006-5G 5 g
EUR 64.06
Description: N/A

0.1g Gentamicin Sulfate Powder

61-098-RA EACH
EUR 58.37

1g Gentamicin Sulfate Powder

61-098-RF EACH
EUR 76.38

Gentamicin Sulfate, Animal-Free

19771-01 1G
EUR 53.2

Gentamicin Sulfate, Animal-Free

19771-14 5G
EUR 168

Gentamicin Sulfate, Animal-Free

19771-72 25G
EUR 630

Gentamicin Sulfate 10 mg/ml - 100ml

LM-A4111/100 100ml
EUR 20.19

Gentamicin Sulfate 50 mg/ml - 100ml

LM-A4112/100 100ml
EUR 30.25

Gentamicin C1 Sulfate EvoPure®

G031-10MG 10 mg
EUR 569.63
Description: C21H43N5O7• xH2SO4 (lot specific)

Gentamicin C2 sulfate EvoPure®

G033-10MG 10 mg
EUR 569.63
Description: C20H41N5O7• xH2SO4(lot specific)

Gentamicin A sulfate EvoPure®

G035-10MG 10 mg
EUR 311.7
Description: C18H36N4O10• xH2SO4 (lot specific)

Gentamicin X2 sulfate EvoPure®

G036-2.5MG 2.5 mg
EUR 36
Description: C19H38N4O10• xH2SO4 (lot specific)

Gentamicin X2 sulfate EvoPure®

G036-PleaseContactusforPricing Please Contact us for Pricing
EUR 36
Description: C19H38N4O10• xH2SO4 (lot specific)

Gentamicin Sulfate 10 mg/ml - 10ml

LM-A4111/10 10ml
EUR 5.5

Gentamicin Sulfate 50 mg/ml - 10ml

LM-A4112/10 10ml
EUR 6.11

Gentamicin Sulfate Solution(10mg/ml)

16672-04 10ML
EUR 16.1

Gentamicin Sulfate Solution(50mg/ml)

11980-14 10ML
EUR 56

10mL Gentamicin Sulfate Liquid 5 - PK10

30-005-CR PK10
EUR 650.7

Gentamicin C1a sulfate EvoPure®

G032-10MG 10 mg
EUR 569.63
Description: C19H39N5O7• xH2SO4 (lot specific)

Gentamicin C2a sulfate EvoPure®

G034-10MG 10 mg
EUR 569.63
Description: C20H41N5O7• xH2SO4 (lot specific)

Gentamicin C2b sulfate EvoPure®

G062-10MG 10 mg
EUR 430.04
Description: C20H41N5O7• xH2SO4 (lot specific)

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-1 20x1 mL
EUR 58.57

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-2 20 mL
EUR 53.99

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-3 50 mL
EUR 77.39

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-4 100 mL
EUR 116.22

Gentamicin Sulfate Solution, 10mg/mL, Sterile

40120796-5 100 mL
EUR 73.7

Gentamicin Sulfate Solution, 100mg/mL, Sterile

40120797-1 20 mL
EUR 59.15

Gentamicin Sulfate Solution, 100mg/mL, Sterile

40120797-2 100 mL
EUR 148.6

Gentamicin Sulfate Solution, 30mg/mL, Sterile

40120798-1 20 mL
EUR 37.61

Gentamicin Sulfate Solution, 30mg/mL, Sterile

40120798-2 100 mL
EUR 75.2

Gentamicin Sulfate Solution, 50mg/mL, Sterile

40710024-1 20 mL
EUR 44.92

Gentamicin Sulfate Solution, 50mg/mL, Sterile

40710024-2 100 mL
EUR 112.16

Gentamicin Sulfate Solution (50 mg/mL in Water)

G046-20ML 20 mL
EUR 69.12
Description: C21H43N5O7

Gentamicin B1 Acetate Salt

G360560 0.5mg
EUR 7738

Gentamicin C1 Pentaacetate Salt

G360585 10mg
EUR 1246

Gentamicin C1 pentaacetate salt

GA1270-1 1
EUR 138.5

Gentamicin C1 pentaacetate salt

GA1270-10 10
EUR 617.8

Gentamicin C1 pentaacetate salt

GA1270-10MG 10 mg
EUR 783.6

Gentamicin C1 pentaacetate salt

GA1270-1MG 1 mg
EUR 204

Gentamicin C2 pentaacetate salt

GA2476-5 5
EUR 316.4

Gentamicin C2 pentaacetate salt

GA2476-5MG 5 mg
EUR 418.8

Gentamicin C1a Pentaacetate Salt

G360590 10mg
EUR 655

Gentamicin C1a pentaacetate salt

GA4559-10 10
EUR 395.5

Gentamicin C1a pentaacetate salt

GA4559-10MG 10 mg
EUR 514.8

Gentamicin C1a pentaacetate salt

GA4559-25 25
EUR 767.1
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