gentatest

modulates the gentamicin activity against multi-resistant strains and induces nefroprotection

by Krystal | Posted on

Dioclea violacea lectin modulates the gentamicin activity against multi-resistant strains and induces nefroprotection during antibiotic exposure.

Gentamicin is an aminoglycoside antibiotic used to treat infections of various origins. In the last few decades, the constant use of gentamicin has resulted in increased bacterial resistance and nephrotoxicity in some cases. In this study, we examined the ability of Dioclea violacea lectin (DVL) in modulate the antimicrobial activity of gentamicin and reduce the nephrotoxicity induced by this drug.

 

The minimum inhibitory concentration (MIC) obtained for DVL against all strains studied was not clinically relevant (MIC ≥ 1,024 μg/mL). However, when DVL was combined with gentamicin, a significant increase in antibiotic action was observed against Staphylococcus aureus and Escherichia coli. DVL also reduced antibiotic tolerance in S. aureus during 10 days of continuous treatment.

 

In addition, DVL presented a nephroprotective effect, reducing sodium excretion, N-Gal expression and urinary protein, that are important markers of glomerular and tubular injuries. Taken together, studies of inhibition of hemagglutinating activity, fluorescence spectroscopy and molecular docking revealed that gentamicin can interact with DVL via the carbohydrate recognition domain (CRD), suggesting that the results obtained in this study may be directly related to the interaction of DVL-gentamicin and with the ability of the lectin to interact with glycans present in the cells of the peritoneum.

Antioxidant, anti-inflammatory, and antiapoptotic effects of virgin coconut oil against antibiotic drug gentamicin-induced nephrotoxicity via the suppression of oxidative stress and modulation of iNOS/NF-ĸB/caspase-3 signaling pathway in Wistar rats.

Gentamicin is an effective antibiotic against severe infections; however, its major side effect is oxidative nephrotoxicity. We explored whether virgin coconut oil (VCO) could mitigate gentamicin-induced nephrotoxicity. Rats were fed with VCO-supplemented diet for 16 days against renal toxicity induced by gentamicin (100 mg/kg bw, ip) from Day 11 to 16. Gentamicin caused marked elevated serum urea, uric acid, and creatinine levels, followed by considerable depletion in renal antioxidant enzymes, glutathione (GSH), while the malondialdehyde (MDA) level increased significantly.

 

  • It significantly increased renal cytokines and nitric oxide (NO) levels, confirmed by renal histopathology. The expression of inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-ĸB), and caspase-3 was prominently increased.
  • VCO-supplemented diet significantly modulated the levels of biochemical indices, downregulated the expression of NO, iNOS, NF-ĸB, caspase-3, cytokines, and alleviated histopathological lesions. VCO protects against gentamicin-induced nephrotoxicity; thus, it could be a promising dietary supplement for patients undergoing gentamicin treatment.
  • PRACTICAL APPLICATIONS: Gentamicin is an efficacious clinical antibiotic used against severe infections; however, the robust body of evidence indicates that the nephrotoxic side effect constrained its use. Virgin coconut oil (VCO) is an edible oil with growing human consumption and pharmacological effects.
  • Our study has reported herein, for the first time, that VCO diet prevented the nephrotoxicity of gentamicin. Dietary supplementation of this oil could be beneficial in alleviating the nephrotoxic side effect of gentamicin in patients.
gentatest
gentatest

 

Gentamicin(GM)
AT022 1mg
EUR 1134

Gentamicin(GM)
AG022 1 mg
EUR 627.6

Gentamicin [KLH]
DAG4484 1mg
EUR 1248

Gentamicin [HRP]
DAGA-032H 1mg
EUR 1248

Gentamicin-OVA
80-1455 1 mg
EUR 781.2
Description: OVA conjugated Gentamicin Hapten

Gentamicin-OVA
80-1456 1 mg
EUR 781.2
Description: OVA conjugated Gentamicin Hapten

Gentamicin-HRP
65-IG10 1 mg
EUR 716.4
Description: Conjugated Gentamicin-HRP hapten

Gentamicin sulfate
B1270-1G each
EUR 170.4

Gentamicin sulfate
B1270-25G each
EUR 1136.4

Gentamicin sulfate
B1270-5G each
EUR 405.6

Gentamicin sulfate
GA7939-10G 10 g
EUR 122.4

Gentamicin sulfate
GA7939-1G 1 g
EUR 55.2

Gentamicin sulfate
GA7939-25G 25 g
EUR 189.6

Gentamicin sulfate
GA7939-5G 5 g
EUR 93.6

Gentamicin (sulfate)
HY-A0276 500mg
EUR 142.8

Gentamicin sulfate
GA7939-1 1
EUR 15.9

Gentamicin sulfate
GA7939-10 10
EUR 71.1

Gentamicin sulfate
GA7939-25 25
EUR 126.5

Gentamicin sulfate
GA7939-5 5
EUR 47.4

Gentamicin Antibody
20-abx210193
  • EUR 526.80
  • EUR 393.60
  • 100 ug
  • 50 ug

Gentamicin antibody
10-1541 100 ug
EUR 470.4
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody
10-G02A 500 ug
EUR 591.6
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody
20C-CR1031S 1 ml
EUR 262.8
Description: Sheep polyclonal Gentamicin antiserum

Gentamicin antibody
20-GG15 250 ul
EUR 220.8
Description: Goat polyclonal Gentamicin antibody

Gentamicin antibody
20-GR15 250 ul
EUR 249.6
Description: Rabbit polyclonal Gentamicin antibody

Gentamicin Antibody
abx021011-200ug 200 ug
EUR 393.6

Gentamicin Antibody
abx021012-1mg 1 mg
EUR 1144.8

Gentamicin Antibody
abx021013-1mg 1 mg
EUR 577.2

Gentamicin, 50mg/ml
CA003-001 10ml
EUR 109.2

Gentamicin ELISA kit
55R-2233 1 kit
EUR 837.6
Description: Gentamicin ELISA kit for use in research laboratory

Gentamicin ELISA Kit
DEIA047 96T
EUR 1238.4
Description: This kit can be used in quantitative and qualitative analysis of gentamicin residue in vaccine and cell culture.

Gentamicin ELISA Kit
K4206-100 each
EUR 1188

Gentamicin Sulfate EP
G007-25G 25 g
EUR 478.8

Gentamicin Sulfate EP
G007-5G 5 g
EUR 219.6

Gentamicin Sulfate USP
G006-25G 25 g
EUR 470.4

Gentamicin Sulfate USP
G006-5G 5 g
EUR 199.2

Sd Gentamicin Gm-10
231299 PK10
EUR 63.45

Gentamicin (GTM) Antibody
20-abx175177
  • EUR 393.60
  • EUR 994.80
  • EUR 526.80
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gentamicin sulfate salt
20-abx082235
  • EUR 226.80
  • EUR 343.20
  • 100 mg
  • 1 g

Gentamicin (GEN) ELISA Kit
RDFS-GEN-48T 48T
EUR 488
Description: Chicken, liver, etc.

Gentamicin (GEN) ELISA Kit
RDFS-GEN-96T 96T
EUR 698
Description: Chicken, liver, etc.

0.1g Gentamicin Sulfate Powder
61-098-RA EACH
EUR 58.37

1g Gentamicin Sulfate Powder
61-098-RF EACH
EUR 76.38

Gentamicin monoclonal antibody
10R-11497 1 mg
EUR 1332
Description: Mouse anti- Gentamicin monoclonal antibody

Gentamicin ELISA Kit (OKAO00127)
OKAO00127 96 Wells
EUR 638.4
Description: Description of target: Gentamicin is a complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.;Species reactivity: General;Application: ;Assay info: Assay Methodology: Competitive Inhibition ELISA;Sensitivity: _x000D__x000D__x000D__x000D_
ComponentAmount
Tissue6 ppb
Liver10 ppb
Milk15 ppb
Serum6 ppb

Gentamicin C1 pentaacetate salt
GA1270-10MG 10 mg
EUR 783.6

Gentamicin C1 pentaacetate salt
GA1270-1MG 1 mg
EUR 204

Gentamicin C2 pentaacetate salt
GA2476-5MG 5 mg
EUR 418.8

Gentamicin C1 pentaacetate salt
GA1270-1 1
EUR 138.5

Gentamicin C1 pentaacetate salt
GA1270-10 10
EUR 617.8

Gentamicin C2 pentaacetate salt
GA2476-5 5
EUR 316.4

Gentamicin C1a pentaacetate salt
GA4559-10MG 10 mg
EUR 514.8

Gentamicin C1a pentaacetate salt
GA4559-25MG 25 mg
EUR 964.8

Gentamicin C1a pentaacetate salt
GA4559-10 10
EUR 395.5

Gentamicin C1a pentaacetate salt
GA4559-25 25
EUR 767.1

Gentamicin C2 sulfate EvoPure®
G033-10MG 10 mg
EUR 804

Gentamicin A sulfate EvoPure®
G035-10MG 10 mg
EUR 456

Gentamicin X2 sulfate EvoPure®
G036-2.5MG 2.5 mg
EUR 36

Gentamicin X2 sulfate EvoPure®
G036-PleaseContactusforPricing Please Contact us for Pricing
EUR 36

Gentamicin (serum/urine) ELISA Kit
K4315-100 each
EUR 907.2

Gentamicin C1 Sulfate EvoPure®
G031-10MG 10 mg
EUR 804

10mL Gentamicin Sulfate Liquid 5
30-005-CR PK10
EUR 578.4

Gentamicin C1a sulfate EvoPure®
G032-10MG 10 mg
EUR 804

Gentamicin C2a sulfate EvoPure®
G034-10MG 10 mg
EUR 804

Gentamicin C2b sulfate EvoPure®
G062-10MG 10 mg
EUR 615.6

GTTR [Texas Red gentamicin conjugate]
24300 100 ug
EUR 203

GTTR [Texas Red gentamicin conjugate]
24301 1 mg
EUR 989

GTTR [Texas Red gentamicin conjugate]
24300-100ug 100 ug
EUR 203
Description: Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units.

GTTR [Texas Red gentamicin conjugate]
24301-1mg 1 mg
EUR 989
Description: Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units.

Sheep Anti Gentamicin Polyclonal Antibody
DPBT-68266SG 0.5 ml
EUR 920.4

Mouse Anti Gentamicin Monoclonal Antibody
DMABT-54913MG 1 mg
EUR 920.4

Mouse Anti Gentamicin Monoclonal Antibody
DMABT-54914MG 1 mg
EUR 920.4

Gentamicin Sulfate Solution (50 mg/mL in Water)
G046-20ML 20 mL
EUR 132

SP-TL Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make SP-TALP (Tyrode's Albumin Lactate Pyruvate).
IVL03-100ML 100 ml
EUR 86.4
Description: Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make SP-TALP (Tyrode's Albumin Lactate Pyruvate).

SP-TL Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make SP-TALP (Tyrode's Albumin Lactate Pyruvate).
IVL03-6X100ML 6 x 100 ml
EUR 126
Description: Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make SP-TALP (Tyrode's Albumin Lactate Pyruvate).

HEPES-TL Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make HEPES-TALP (Tyrode's Albumin Lactate Pyruvate).
IVL01-500ML 500 ml
EUR 104.4
Description: Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make HEPES-TALP (Tyrode's Albumin Lactate Pyruvate).

HEPES-TL Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make HEPES-TALP (Tyrode's Albumin Lactate Pyruvate).
IVL01-6X500ML 6 x 500 ml
EUR 226.8
Description: Supplement with BSA (Fract V), sodium pyruvate, and gentamicin to make HEPES-TALP (Tyrode's Albumin Lactate Pyruvate).

IVF-TL Supplement with BSA (EFAF), sodium pyruvate, gentamicin, and heparin to make IVF-TALP (Tyrode's Albumin Lactate Pyruvate).
IVL02-100ML 100 ml
EUR 90
Description: Supplement with BSA (EFAF), sodium pyruvate, gentamicin, and heparin to make IVF-TALP (Tyrode's Albumin Lactate Pyruvate).

 

Gentamicin, azithromycin and ceftriaxone in the treatment of gonorrhoea: the relationship between antibiotic MIC and clinical outcome.

To investigate the relationship between MIC and clinical outcome in a randomized controlled trial that compared gentamicin 240 mg plus azithromycin 1 g with ceftriaxone 500 mg plus azithromycin 1 g. MIC analysis was performed on Neisseria gonorrhoeae isolates from all participants who were culture positive before they received treatment.

Viable gonococcal cultures were available from 279 participants, of whom 145 received ceftriaxone/azithromycin and 134 received gentamicin/azithromycin. Four participants (6 isolates) and 14 participants (17 isolates) did not clear infection in the ceftriaxone/azithromycin and gentamicin/azithromycin arms, respectively. MICs were determined by Etest on GC agar base with 1% Vitox.

 

The geometric mean MICs of azithromycin, ceftriaxone and gentamicin were compared using logistic and linear regression according to treatment received and N. gonorrhoeae clearance.As the azithromycin MIC increased, gentamicin/azithromycin treatment was less effective than ceftriaxone/azithromycin at clearing N. gonorrhoeae.

 

There was a higher geometric mean MIC of azithromycin for isolates from participants who had received gentamicin/azithromycin and did not clear infection compared with those who did clear infection [ratio 1.95 (95% CI 1.28-2.97)], but the use of categorical MIC breakpoints did not accurately predict the treatment response.

 

The geometric mean MIC of azithromycin was higher in isolates from the pharynx compared with genital isolates.We found that categorical resistance to azithromycin or ceftriaxone in vitro, and higher gentamicin MICs in the absence of breakpoints, were poorly predictive of treatment failure.

 

 

Gentamicin sulfate
GA7939-25G 25 g
EUR 189.6

Gentamicin sulfate
GA7939-5G 5 g
EUR 93.6

Gentamicin (sulfate)
HY-A0276 500mg
EUR 142.8

Gentamicin sulfate
GA7939-1 1
EUR 15.9

Gentamicin sulfate
GA7939-10 10
EUR 71.1

Gentamicin sulfate
GA7939-25 25
EUR 126.5

Gentamicin sulfate
GA7939-5 5
EUR 47.4

Gentamicin Sulfate EP
G007-25G 25 g
EUR 478.8

Gentamicin Sulfate EP
G007-5G 5 g
EUR 219.6

Gentamicin Sulfate USP
G006-25G 25 g
EUR 470.4

Gentamicin Sulfate USP
G006-5G 5 g
EUR 199.2

0.1g Gentamicin Sulfate Powder
61-098-RA EACH
EUR 58.37

1g Gentamicin Sulfate Powder
61-098-RF EACH
EUR 76.38

Gentamicin C2 sulfate EvoPure®
G033-10MG 10 mg
EUR 804

Gentamicin A sulfate EvoPure®
G035-10MG 10 mg
EUR 456

Gentamicin X2 sulfate EvoPure®
G036-2.5MG 2.5 mg
EUR 36

Gentamicin X2 sulfate EvoPure®
G036-PleaseContactusforPricing Please Contact us for Pricing
EUR 36

Gentamicin C1 Sulfate EvoPure®
G031-10MG 10 mg
EUR 804

10mL Gentamicin Sulfate Liquid 5
30-005-CR PK10
EUR 578.4

Gentamicin C1a sulfate EvoPure®
G032-10MG 10 mg
EUR 804

Gentamicin C2a sulfate EvoPure®
G034-10MG 10 mg
EUR 804

Gentamicin C2b sulfate EvoPure®
G062-10MG 10 mg
EUR 615.6

Gentamicin Sulfate Solution (50 mg/mL in Water)
G046-20ML 20 mL
EUR 132

Gentamicin C1 pentaacetate salt
GA1270-10MG 10 mg
EUR 783.6

Gentamicin C1 pentaacetate salt
GA1270-1MG 1 mg
EUR 204

Gentamicin C2 pentaacetate salt
GA2476-5MG 5 mg
EUR 418.8

Gentamicin C1 pentaacetate salt
GA1270-1 1
EUR 138.5

Gentamicin C1 pentaacetate salt
GA1270-10 10
EUR 617.8

Gentamicin C2 pentaacetate salt
GA2476-5 5
EUR 316.4

Gentamicin C1a pentaacetate salt
GA4559-10MG 10 mg
EUR 514.8

Gentamicin C1a pentaacetate salt
GA4559-25MG 25 mg
EUR 964.8

Gentamicin C1a pentaacetate salt
GA4559-10 10
EUR 395.5

Gentamicin C1a pentaacetate salt
GA4559-25 25
EUR 767.1

Gentamicin Solution, Gentamicin Solution, 50 mg/mL
CCM1123-010 10 mL
EUR 76.86

Gentamicin(GM)
AT022 1mg
EUR 1134

Gentamicin(GM)
AG022 1 mg
EUR 627.6

Gentamicin [KLH]
DAG4484 1mg
EUR 1248

Gentamicin [HRP]
DAGA-032H 1mg
EUR 1248

Gentamicin-BSA
80-1453 1 mg
EUR 781.2
Description: BSA conjugated Gentamicin Hapten

Gentamicin-BSA
80-1454 1 mg
EUR 781.2
Description: BSA conjugated Gentamicin Hapten

Gentamicin-OVA
80-1455 1 mg
EUR 781.2
Description: OVA conjugated Gentamicin Hapten

Gentamicin-OVA
80-1456 1 mg
EUR 781.2
Description: OVA conjugated Gentamicin Hapten

Gentamicin-BSA
80-IG10 25 mg
EUR 457.2
Description: Conjugated Gentamicin-BSA hapten

Gentamicin-HRP
65-IG10 1 mg
EUR 716.4
Description: Conjugated Gentamicin-HRP hapten

Gentamicin Antibody
20-abx210193
  • EUR 526.80
  • EUR 393.60
  • 100 ug
  • 50 ug

Gentamicin antibody
10-1541 100 ug
EUR 470.4
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody
10-G02A 500 ug
EUR 591.6
Description: Mouse monoclonal Gentamicin antibody

Gentamicin antibody
20C-CR1031S 1 ml
EUR 262.8
Description: Sheep polyclonal Gentamicin antiserum

Gentamicin antibody
20-GG15 250 ul
EUR 220.8
Description: Goat polyclonal Gentamicin antibody

Gentamicin antibody
20-GR15 250 ul
EUR 249.6
Description: Rabbit polyclonal Gentamicin antibody

Gentamicin Antibody
abx021011-200ug 200 ug
EUR 393.6

Gentamicin Antibody
abx021012-1mg 1 mg
EUR 1144.8

Gentamicin Antibody
abx021013-1mg 1 mg
EUR 577.2

Gentamicin, 50mg/ml
CA003-001 10ml
EUR 109.2

Gentamicin ELISA kit
55R-2233 1 kit
EUR 837.6
Description: Gentamicin ELISA kit for use in research laboratory

Gentamicin ELISA Kit
DEIA047 96T
EUR 1238.4
Description: This kit can be used in quantitative and qualitative analysis of gentamicin residue in vaccine and cell culture.

Gentamicin ELISA Kit
K4206-100 each
EUR 1188

Sd Gentamicin Gm-10
231299 PK10
EUR 63.45

Gentamicin (GTM) Antibody
20-abx175177
  • EUR 393.60
  • EUR 994.80
  • EUR 526.80
  • EUR 184.80
  • EUR 309.60
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Gentamicin (GEN) ELISA Kit
RDFS-GEN-48T 48T
EUR 488
Description: Chicken, liver, etc.

Gentamicin (GEN) ELISA Kit
RDFS-GEN-96T 96T
EUR 698
Description: Chicken, liver, etc.

Gentamicin monoclonal antibody
10R-11497 1 mg
EUR 1332
Description: Mouse anti- Gentamicin monoclonal antibody

Gentamicin ELISA Kit (OKAO00127)
OKAO00127 96 Wells
EUR 638.4
Description: Description of target: Gentamicin is a complex of closely related aminoglycosides obtained from MICROMONOSPORA purpurea and related species. They are broad-spectrum antibiotics, but may cause ear and kidney damage. They act to inhibit PROTEIN BIOSYNTHESIS.;Species reactivity: General;Application: ;Assay info: Assay Methodology: Competitive Inhibition ELISA;Sensitivity: _x000D__x000D__x000D__x000D_
ComponentAmount
Tissue6 ppb
Liver10 ppb
Milk15 ppb
Serum6 ppb

Gentamicin (serum/urine) ELISA Kit
K4315-100 each
EUR 907.2

GTTR [Texas Red gentamicin conjugate]
24300 100 ug
EUR 203

GTTR [Texas Red gentamicin conjugate]
24301 1 mg
EUR 989

GTTR [Texas Red gentamicin conjugate]
24300-100ug 100 ug
EUR 203
Description: Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units.

GTTR [Texas Red gentamicin conjugate]
24301-1mg 1 mg
EUR 989
Description: Gentamicin is widely used for treating tuberculosis and Gram-negative infections and is particularly useful in neonatal intensive care units.

Sheep Anti Gentamicin Polyclonal Antibody
DPBT-68266SG 0.5 ml
EUR 920.4

Mouse Anti Gentamicin Monoclonal Antibody
DMABT-54913MG 1 mg
EUR 920.4

Mouse Anti Gentamicin Monoclonal Antibody
DMABT-54914MG 1 mg
EUR 920.4

Gentamycin Sulfate
A2514-1000 1 g
EUR 166.8
Description: Gentamicin is an aminoglycoside antibiotic, always used the sulfate form, composed of a mixture of related gentamicin components and fractions ,which is used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms.

Gentamycin Sulfate
A2514-5.1 10 mM (in 1mL DMSO)
EUR 129.6
Description: Gentamicin is an aminoglycoside antibiotic, always used the sulfate form, composed of a mixture of related gentamicin components and fractions ,which is used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms.

Gentamycin Sulfate, 100g
R1405-100g each
EUR 649.2

TruStrip RDT Gentamicin rapid test strips, results is 2-10 mins, 50 strips/pk
DE-100200-RDT 1 pk Ask for price

Gentamycin Sulfate, 25g
R1405-25g each
EUR 228

Rat  Gentamicin (GTM) kit ELISA kit
E02G0015-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat  Gentamicin (GTM) kit in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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